The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions ended up digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the short adjust https://gw-406488654.wizzardsblog.com/26580382/not-known-facts-about-fk-330-dihydrate